Intersection · Analytics × system suitability

Analytics topics tagged system suitability

Topics in Analytics carrying the tag system suitability. The injections that establish whether a run was valid at all.

47 topics

Topic	Participants	Replies	Views	Activity
What a reversed-phase purity number actually is HPLC system suitability data table peak integration Solved The question in the title: What a reversed-phase purity number actually is I will give what I have already checked below so nobody repeats it. I would like to understand what this number means before I repeat…	+87	101	27k	4h
Repeatability versus intermediate precision — what changed since recovery precision & repeatability linearity system suitability Posting this under the heading it deserves: Repeatability versus intermediate precision — what changed since Everything below is what sits behind that. What changes if the standard account of Repeatability…		4	21k	5h
[2026 update] Detection wavelength and why 214 nm and 280 nm disagree HPLC system suitability gradient method Detection wavelength and why 214 nm and 280 nm disagree — setting out what I have, and where I think it stops being reliable. I would like to disagree carefully with the settled view on Detection wavelength,…	+69	76	920	6h
Follow-up: Comparing two chromatograms from different laboratories, properly gradient method system suitability HPLC peak integration Comparing two chromatograms from different laboratories, properly — setting out what I have, and where I think it stops being reliable. I would like to understand what this number means before I repeat it…	+62	67	12k	9h
Why 99.2% and 97.8% on the same lot can both be correct system suitability peak integration gradient method data table Solved Why 99.2% and 97.8% on the same lot can both be correct I have a specific reason for asking rather than idle curiosity, and the context is below. I would like to understand what this number means before I…	+16	20	3.1k	11h
Shoulders, tangent skims, and defensible integration peak integration UV & DAD detection HPLC gradient method Posting this under the heading it deserves: Shoulders, tangent skims, and defensible integration Everything below is what sits behind that. Setting out an integration problem with the actual figures, because…	+16	20	11k	13h
Revisiting: Spike-and-recover accuracy for a peptide assay LOD & LOQ system suitability stability-indicating method linearity Revisiting: Spike-and-recover accuracy for a peptide assay — setting out what I have, and where I think it stops being reliable. I was wrong about Spike-and-recover accuracy in a thread last spring and I…		2	6.8k	21h
Gradient steepness and apparent purity: a worked comparison — the long version HPLC system suitability gradient method Gradient steepness and apparent purity: a worked comparison — the long version — setting out what I have, and where I think it stops being reliable. A narrow question about Gradient steepness and apparent…		2	32k	1d
System suitability injections: if they failed, the run did not happen — the long version gradient method UV & DAD detection system suitability peak integration System suitability injections: if they failed, the run did not happen — the long version — setting out what I have, and where I think it stops being reliable. A narrow question about System suitability…	+2	6	6.2k	1d
Revisiting: Theoretical plates: useful number or ritual? system suitability UV & DAD detection gradient method data table Solved Asking directly, because I could not find a straight answer: Revisiting: Theoretical plates: useful number or ritual? Asking about Theoretical plates directly, because I have read four threads on it and each…	+16	21	1.2k	1d
[2026 update] Why 99.2% and 97.8% on the same lot can both be correct UV & DAD detection system suitability peak integration gradient method Solved Why 99.2% and 97.8% on the same lot can both be correct — that is the question, and I have not found it answered plainly anywhere I have looked. I would like to understand what this number means before I…	+1	5	719	2d
Coming back to: Specificity, linearity, accuracy, precision, range, robustness — with real criteria linearity recovery precision & repeatability system suitability On the subject in the title: Specificity, linearity, accuracy, precision, range, robustness — with real criteria Working notes rather than a conclusion. Trying to establish the boundary conditions on…	+93	99	1.4k	6d
Carryover and the ghost peak from last week's standard — what changed since gradient method data table HPLC UV & DAD detection Solved Carryover and the ghost peak from last week's standard — what changed since Writing it up because I had to work it out twice and would rather nobody else did. What changes if the standard account of Carryover…	+98	114	37k	22d
Resolution and tailing factor: real acceptance criteria HPLC gradient method peak integration system suitability Resolution and tailing factor: real acceptance criteria Writing it up because I had to work it out twice and would rather nobody else did. Asking about resolution and tailing factor on behalf of the question…		2	15k	1mo
Injection volume, overload, and peak distortion peak integration system suitability HPLC data table Solved Posting this under the heading it deserves: Injection volume, overload, and peak distortion Everything below is what sits behind that. I have spent a fortnight trying to pin injection volume down and I want…	+43	47	36k	2mo
Area percent versus weight percent: the confusion that causes most arguments system suitability UV & DAD detection HPLC data table Posting this under the heading it deserves: Area percent versus weight percent: the confusion that causes most arguments Everything below is what sits behind that. Proposing that area percent versus weight…	+10	14	2.1k	2mo
Validating a method you did not develop precision & repeatability recovery linearity stability-indicating method On the subject in the title: Validating a method you did not develop Working notes rather than a conclusion. What changes if the standard account of validating a method is wrong? I ask because I have been…	+10	14	15k	3mo
Sample handling between despatch and injection stability-indicating method LOD & LOQ linearity precision & repeatability Sample handling between despatch and injection Writing it up because I had to work it out twice and would rather nobody else did. Trying to work out what would count as evidence on sample handling between…	+63	68	2.4k	3mo
Second pass at: Robustness: the parameters worth deliberately varying linearity LOD & LOQ recovery system suitability On the subject in the title: Second pass at: Robustness: the parameters worth deliberately varying Working notes rather than a conclusion. Something about Robustness does not reconcile and I would like a…	+61	66	9.5k	3mo
Shoulders, tangent skims, and defensible integration — the long version HPLC system suitability peak integration data table Shoulders, tangent skims, and defensible integration — the long version Writing it up because I had to work it out twice and would rather nobody else did. Setting out an integration problem with the actual…	+16	20	27k	3mo
Spike-and-recover accuracy for a peptide assay linearity system suitability stability-indicating method LOD & LOQ Wiki Posting this under the heading it deserves: Spike-and-recover accuracy for a peptide assay Everything below is what sits behind that. Asking about spike-and-recover accuracy on behalf of the question I keep…	+121	126	1.5k	4mo
Injection volume, overload, and peak distortion — one year on system suitability peak integration gradient method data table Under review Injection volume, overload, and peak distortion — one year on Writing it up because I had to work it out twice and would rather nobody else did. Posting a small dataset on Injection volume. It is mine, it is…	+4	8	1.2k	4mo
Why one laboratory's 98.4% and another's 96.1% may not contradict — one year on LOD & LOQ system suitability linearity stability-indicating method Asking directly, because I could not find a straight answer: Why one laboratory's 98.4% and another's 96.1% may not contradict — one year on Posting the method first, because I know what the first three…	+10	14	13k	5mo
Revisiting: Sample handling between despatch and injection recovery precision & repeatability linearity LOD & LOQ Posting this under the heading it deserves: Revisiting: Sample handling between despatch and injection Everything below is what sits behind that. Reading back through what has been written here about Sample…	+5	9	1.1k	6mo
An inter-laboratory comparison on one lot, tabulated — what changed since stability-indicating method LOD & LOQ system suitability precision & repeatability An inter-laboratory comparison on one lot, tabulated — what changed since — setting out what I have, and where I think it stops being reliable. What changes if the standard account of inter-laboratory…	+24	28	3.3k	7mo
Comparing two chromatograms from different laboratories, properly peak integration system suitability UV & DAD detection gradient method Solved Closed Posting this under the heading it deserves: Comparing two chromatograms from different laboratories, properly Everything below is what sits behind that. I would like to understand what this number means…	+35	40	22k	7mo
Where the integrator drew the baseline, and how much it moved the number system suitability gradient method UV & DAD detection data table Solved Where the integrator drew the baseline, and how much it moved the number — setting out what I have, and where I think it stops being reliable. Posting the method first, because I know what the first three…	+20	24	1.6k	8mo
What a reversed-phase purity number actually is — the long version gradient method system suitability HPLC UV & DAD detection Solved What a reversed-phase purity number actually is — the long version I have a specific reason for asking rather than idle curiosity, and the context is below. I would like to understand what this number means…		2	15k	8mo
LOD and LOQ: definitions people get wrong precision & repeatability recovery stability-indicating method linearity Closed LOD and LOQ: definitions people get wrong — setting out what I have, and where I think it stops being reliable. What changes if the standard account of LOD and LOQ is wrong? I ask because I have been treating…		2	43k	8mo
Detection wavelength and why 214 nm and 280 nm disagree peak integration UV & DAD detection system suitability HPLC On the subject in the title: Detection wavelength and why 214 nm and 280 nm disagree Working notes rather than a conclusion. I would like to disagree carefully with the settled view on detection wavelength,…	+78	84	1.7k	8mo

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