Tags · Technique
endotoxin testing
Bacterial endotoxin testing, LAL and rFC methods.
Related in this group: HPLC · LC-MS · high-resolution MS · MALDI-TOF · UV & DAD detection · peak integration · system suitability · gradient method · water content · amino acid analysis · ELISA · sterility testing
19 topics
| Topic | Participants | Replies | Views | Activity |
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Deletion sequences: expected mass shifts by residue — the long version
On the subject in the title: Deletion sequences: expected mass shifts by residue — the long version Working notes rather than a conclusion. Deletion sequences keeps being re-asked here and I think that is…
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+21 | 25 | 6.9k | 10h |
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Endotoxin and why it is a separate question from purity — what changed since
On the subject in the title: Endotoxin and why it is a separate question from purity — what changed since Working notes rather than a conclusion. What changes if the standard account of Endotoxin is wrong? I…
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+78 | 91 | 1k | 10h |
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Impurity thresholds: where the common numbers come from
Impurity thresholds: where the common numbers come from — setting out what I have, and where I think it stops being reliable. I have spent a fortnight trying to pin impurity thresholds down and I want to set…
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+11 | 15 | 825 | 2d |
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Deamidation and the close-eluting pair it produces — the long version
Posting this under the heading it deserves: Deamidation and the close-eluting pair it produces — the long version Everything below is what sits behind that. A narrow question about Deamidation, deliberately…
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3 | 347 | 24d | |
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Second pass at: Where impurities in solid-phase peptide synthesis come from
Second pass at: Where impurities in solid-phase peptide synthesis come from Writing it up because I had to work it out twice and would rather nobody else did. Impurities in solid-phase peptide synthesis, from…
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+4 | 8 | 7.2k | 1mo |
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Racemisation and why it is invisible to a mass measurement
Posting this under the heading it deserves: Racemisation and why it is invisible to a mass measurement Everything below is what sits behind that. A question about racemisation that I think has a definite…
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+120 | 127 | 41k | 1mo |
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Residual solvents and scavengers: what is looked for — what changed since
Residual solvents and scavengers: what is looked for — what changed since — setting out what I have, and where I think it stops being reliable. What changes if the standard account of Residual solvents and…
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+37 | 41 | 7k | 2mo |
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Named, unnamed and unspecified impurities as regulatory categories
On the subject in the title: Named, unnamed and unspecified impurities as regulatory categories Working notes rather than a conclusion. Two reports on the same lot, and I would like help deciding whether they…
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+10 | 14 | 504 | 3mo |
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Impurity thresholds: where the common numbers come from — what changed since
Posting this under the heading it deserves: Impurity thresholds: where the common numbers come from — what changed since Everything below is what sits behind that. What changes if the standard account of…
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2 | 61k | 3mo | |
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Deletion sequences: expected mass shifts by residue
Deletion sequences: expected mass shifts by residue — setting out what I have, and where I think it stops being reliable. Deletion sequences keeps being re-asked here and I think that is because the answer is…
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2 | 59 | 3mo | |
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Where impurities in solid-phase peptide synthesis come from
Where impurities in solid-phase peptide synthesis come from Writing it up because I had to work it out twice and would rather nobody else did. Impurities in solid-phase peptide synthesis, and specifically the…
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+54 | 59 | 14k | 3mo |
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Second pass at: Where impurities in solid-phase peptide synthesis come from
Posting this under the heading it deserves: Second pass at: Where impurities in solid-phase peptide synthesis come from Everything below is what sits behind that. Working notes on impurities in solid-phase…
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+47 | 51 | 3.3k | 5mo |
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[2026 update] A rising related-substance total over six months: degradation or method drift?
The question in the title: A rising related-substance total over six months: degradation or method drift? I will give what I have already checked below so nobody repeats it. Posting the method first, because…
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+71 | 78 | 34k | 5mo |
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Revisiting: Aggregates and why a purity assay may miss them entirely
Posting this under the heading it deserves: Revisiting: Aggregates and why a purity assay may miss them entirely Everything below is what sits behind that. I would like to disagree carefully with the settled…
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+48 | 53 | 918 | 8mo |
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Particulates in a reconstituted solution: identifying the likely source
Posting this under the heading it deserves: Particulates in a reconstituted solution: identifying the likely source Everything below is what sits behind that. Something about particulates does not reconcile…
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+15 | 19 | 13k | 9mo |
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Follow-up: Attributing a new peak: degradation product or excipient?
Attributing a new peak: degradation product or excipient? I have a specific reason for asking rather than idle curiosity, and the context is below. A follow-up question about Attributing a new peak that I did…
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+15 | 19 | 671 | 17mo |
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Oxidation pathways for methionine and tryptophan
Posting this under the heading it deserves: Oxidation pathways for methionine and tryptophan Everything below is what sits behind that. Working notes on oxidation pathways for methionine rather than a…
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+58 | 63 | 1k | 19mo |
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Follow-up: Trifluoroacetate content and its consequences
On the subject in the title: Trifluoroacetate content and its consequences Working notes rather than a conclusion. A follow-up question about Trifluoroacetate content that I did not know to ask the first…
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+12 | 16 | 5.7k | 21mo |
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A rising related-substance total over six months: degradation or method drift?
Asking directly, because I could not find a straight answer: A rising related-substance total over six months: degradation or method drift? Rising related-substance — I have the observation and I do not trust…
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+37 | 42 | 37k | 23mo |